Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) Powstawanie zarodków w kulturach mikrospor i pylników buraka czerwonego (Beta vulgaris L. subsp. vulgaris)
نویسندگان
چکیده
So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor plants with gibberellin increased number of obtained androgenic embryos. The highest percentage (80%) of microspores at uninuclear stage appeared in buds with 1.3-1.5 mm. The B5 medium with 100 g·L -1 sucrose and 0.1 mg·L 2,4-D (2, 4dichlorophenoxyacetic acid) proved to be better for inducing androgenesis than MS medium supplemented with 0.2 mg·L BAP (6-benzylaminopurine) and 0.5 mg·L IAA (indole-3-acetic acid). First androgenic embryos were placed on B5 medium without plant growth regulators and then on MS medium containing 0.2 mg·L BAP and 1 mg·L NAA (α-naphthaleneacetic acid). Androgenic embryos died on B5 regeneration medium without plant growth regulators. On MS medium first shoots and callus with and without roots were obtained. Rosettes withered during following passages whereas callus tissue developed further. The quantity of DNA in this tissue equivalent to 4X chromosomes.
منابع مشابه
rRNA distribution during microspore development in anthers of Beta vulgaris L. quantitative in situ hybridization analysis.
We related changes in the ultrastructural organization of the nucleoli with the results of quantitative in situ hybridizations to characterize rRNA metabolism during the development of microspore mother cells in the sugar beet anther (Beta vulgaris L.). In the course of meiotic prophase and early postmeiotic interphase the morphological characteristics of the nucleoli are typical of low or no t...
متن کاملEffects of swing date,year and location of seed production on root yield and sugar content in triploid and tetraploid sugar beet (beta vulgaris L.)cultivars at two different locations
متن کامل
Effects ofpollinator line characteristics on quantity and quality of monogerm hybrid seed production in sugar beet (Beta vulgaris L.)
A two-year experiment was carried out to study the effects of pollinator line characteristicson the quantity and quality of monogerm hybrid seed production in sugar beet (Beta vulgaris L.)and select proper pollinator for five promising sugar beet cytoplasmic male sterile lines (CMSs)during 2012-2013 growing seasons. In this study, four diploid pollinator lines were crossed byfive CMSs of sugar ...
متن کاملPositive correlation between recombination rates and levels of genetic variation in natural populations of sea beet (Beta vulgaris subsp. maritima).
The relation between the level of genetic variation and the rate of recombination per physical unit was investigated in sea beet (Beta vulgaris subsp. maritima). The rate of recombination per physical unit was estimated indirectly through marker density in an RFLP linkage map of sugar beet. From this map, we also selected RFLP markers covering two of the nine chromosomes in Beta. The markers we...
متن کاملIdentification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants.
The RNA3-encoded p25 protein of beet necrotic yellow vein virus (BNYVV) is responsible for the production of rhizomania symptoms of sugar beet roots (Beta vulgaris subsp. vulgaris). Here, it was found that the presence of the p25 protein is also associated with the resistance response in rub-inoculated leaves of sugar beet and wild beet (Beta vulgaris subsp. maritima) plants. The resistance phe...
متن کامل